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Table II.

Relative proportions (%)a of phospholipidsb measured in buttermilk solids obtained from raw or pasteurized cream and in the fractions obtained by microfiltration (MF permeate and MF retentate).

Fraction GluCer LacCer PE PI PS PC SM
From raw cream
Buttermilk 0.7 4.3 36.6 6.9 2.5 33.4 12.1
MF permeate 0.3 9.0 29.0 6.7 1.4 37.6 11.5
MF retentate 0.4 6.7 36.7 6.1 2.7 32.0 11.7
From pasteurized cream
Buttermilk 0.6 1.6 39.9 7.1 3.6 30.7 13.0
MF permeate 0.1 2.6 30.4 7.5 2.4 38.8 13.5
MF retentate 0.2 1.2 33.6 7.1 2.5 32.5 13.6
SEM (n = 4) 0.2 0.9 1.9 0.4 0.4 1.3 0.5
Comparison Significance (P value)

Replicate batches N.S.c 0.0446 N.S. 0.0008 N.S. N.S. 0.0103
Heat treatment N.S. < 0.0001 N.S. 0.0248 N.S. N.S. 0.0015
Fractionation (MF) N.S. N.S. 0.0045 N.S. N.S. 0.0008 N.S.
 – (Buttermilk) × (Permeate & Retentate) N.S. N.S. 0.0097 N.S. N.S. 0.0324 N.S.
 – (Permeate) × (Retentate) N.S. N.S. 0.0113 N.S. N.S. 0.0005 N.S.
(Heat treatment) × (MF fractions) N.S. N.S. N.S. N.S. N.S. N.S. N.S.
a

Individual peak area relative to the total HPLC-ELSD chromatogram surface area.

b

GluCer = glucosylceramide; LacCer = lactosylceramide; PE = phosphatidylethanolamine; PI = phosphatidylinositol; PS = phosphatidylserine; PC = phosphatidylcholine; SM = sphingomyelin.

c

N.S. = non-significant (P > 0.05).