Screening for the potential probiotic yeast strains from raw milk to assimilate cholesterol

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Table IV.

Cholesterol assimilation of various yeast strains inoculated in YPD broth, supplemented with bile salt (0.3%) and cholesterol (224.2 μg·mL⁻¹), and incubated at 37 °C from 12 to 72 h.

Strains¹	Cholesterol assimilation (%)²
Strains¹	12 h	24 h	48 h	72 h
BY1	7.5 ± 0.8^de	23.1 ± 1.9^f	36.3 ± 1.1^de	44.0 ± 2.4^d
BY2	2.3 ± 1.6^b	7.0 ± 0.4^b	16.7 ± 1.6^b	23.8 ± 1.5^c
BY5	5.7 ± 1.5^cde	17.9 ± 2.0^e	35.6 ± 1.8^d	40.3 ± 2.1^d
BY6	0.0^a	6.8 ± 0.4^b	17.5 ± 0.3^b	23.5 ± 2.3^c
BY10	17.5 ± 1.3^f	27.0 ± 2.6^g	37.4 ± 2.8^f	43.2 ± 1.9^d
BY15	6.2 ± 0.1^c	13.1 ± 1.1^c	45.7 ± 2.6^g	44.4 ± 3.0^d
BY31	8.7 ± 0.4^e	13.6 ± 1.3^c	44.4 ± 2.4^g	44.4 ± 2.3^d
HY1	0.0^a	0.0^a	0.0^a	5.6 ± 0.2^ab
HY4	0.0^a	13.7 ± 2.1^c	37.4 ± 1.8^f	39.8 ± 2.3^d
HY15	0.0^a	0.0^a	0.0^a	7.2 ± 1.1^b
HJ1	0.0^a	0.0^a	0.0^a	6.5 ± 0.2^ab
HJ8	0.0^a	0.0^a	0.0^a	3.6 ± 0.3^a
HJ2	0.0^a	0.0^a	0.0^a	6.2 ± 0.5^ab
HJ6	0.0^a	0.0^a	0.0^a	5.5 ± 0.4^ab
HJ10	6.2 ± 1.8^cde	22.3 ± 1.6^f	30.9 ± 1.1^c	45.5 ± 2.3^d
HJ15	0.0^a	0.0^a	0.0^a	4.6 ± 0.3^ab
HJ16	0.0^a	0.0^a	0.0^a	6.8 ± 1.1^ab
HJ22	0.0^a	0.0^a	0.0^a	5.4 ± 0.4^ab
HY18	0.0^a	0.0^a	0.0^a	3.7 ± 0.2^ab
CICC1850	5.2 ± 1.5^cd	16.8 ± 2.1^d	36.5 ± 1.4^e	41.5 ± 2.3^d

The names of species are given in Table I.

Results are expressed as mean ± standard deviation.

^a–g

Means within a column with the same letter are not significantly different (P > 0.05).