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Table II.

Effect of bile salt concentration on the growth of selected yeast strains cultured in YPD at 25 °C after 72 h incubation.

Strains Increment1 in the presence of bile salt % (w/v)2
0.1 0.3 0.5
Galactomyces sp.
BY1
Geotrichum sp.
BY2 43.9 ± 1.3 45.0 ± 3.0 55.3 ± 4.1
P. fermentans
BY5 30.9 ± 2.1 10.3 ± 1.3 9.5 ± 2.3
HJ15 56.6 ± 2.7 79.0 ± 2.5 9.2 ± 3.7
HJ16 15.5 ± 2.3 46.5 ± 1.6 54.9 ± 2.4
Trichosporon sp.
BY6 12.4 ± 3.6 10.7 ± 0.6 8.7 ± 0.4
P. kudriavzevii
BY10 60.8 ± 1.2 25.9 ± 1.2 16.1 ± 2.5
BY15 41.8 ± 2.4 21.9 ± 2.3 18.9 ± 3.1
P. guilliermondii
BY31 15.6 ± 0.3 59.3 ± 2.6 81.4 ± 2.7
HY1 24.4 ± 2.4 35.2 ± 2.0 21.9 ± 1.4
HY18 92.8 ± 1.4 90.5 ± 5.0 100.0
R. mucilaginosa
HJ22 8.5 ± 2.6 15.8 ± 1.8 100.0
Y. lipolytica
HY4 13.7 ± 2.6 12.7 ± 1.8 15.9 ± 1.0
HY15 11.7 ± 3.8 18.1 ± 3.3 1.2 ± 0.4
HJ6 64.6 ± 2.4 62.9 ± 2.4 56.6 ± 1.0
HJ8 15.7 ± 3.2 36.8 ± 4.3 43.3 ± 2.9
HJ10 11.9 ± 0.2 11.9 ± 0.8 4.8 ± 1.3
T. gracile
HJ1
HJ2 100.0 100.0 100.0
S. boulardii
CICC1850 100.0 100.0 100.0
1

Bile tolerance (%) = (increment of cells in broth with bile/increment of cells in broth without bile) × 100; – means cannot tolerate bile; and 100% means that the growth rate of yeasts is not affected by the added bile salt at all.

2

All results are expressed as mean ± standard deviation.