Free Access

Table IV.

The scavenging activities of casein hydrolysates and six casein plasteins on DPPH radical and ABTS·+.

Samples The decrease of free amino groups (μmol·g−1 proteins) Scavenging activity on DPPH radicale
Scavenging activity on ABTS·+ f
TEAC (mmol·g−1 proteins) IC50 (μg·mL−1) TEAC (μmol·g−1 proteins) IC50 (μg·mL−1)
Casein hydrolysatesg 0 10.45 ± 0.64b 1854.46 ± 109.54 8.33 ± 1.14d 965.52 ± 56.77
Casein plasteins 1 7.07 76.50 ± 0.30a 253.29 ± 0.98 16.37 ± 1.97a 491.23 ± 24.58
Casein plasteins 2 10.87 73.41 ± 1.13a 263.95 ± 4.02 16.17 ± 2.29c 497.37 ± 52.74
Casein plasteins 3 18.43 77.47 ± 0.27a 250.14 ± 0.86 16.45 ± 1.21a 489.05 ± 7.70
Casein plasteins 4 78.95 86.58 ± 0.57a 223.81 ± 1.48 15.51 ± 0.12b 518.52 ± 1.60
Casein plasteins 5 80.50 84.73 ± 2.16a 228.72 ± 5.91 15.09 ± 0.62b 538.54 ± 9.01
Casein plasteins 6 81.01 90.31 ± 1.42a 214.57 ± 3.36 15.23 ± 1.09b 528.30 ± 15.94
a–d

Data in the same column with different superscript letters differ significantly (P < 0.05) by Duncan’s multiple range tests.

e

The final concentration of casein hydrolysates or six casein plasteins for the assay of scavenging activity on DPPH radical was 100 μg·mL−1.

f

The final concentration of casein hydrolysates or six casein plasteins for the assay of scavenging activity on ABTS·+ was 100 μg·mL−1.

g

The content of free amino groups was 981.7 μmol·g−1 proteins and degree of hydrolysis was 9.6%.