Different proteomic profiles of sweet whey and rennet casein obtained after preparation from raw versus heat-treated skimmed milk

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Table IV.

Proteins in sweet whey fraction (Fig. 3) identified by MALDI TOF MS after non-reducing (−) 2-DGE.

Spot ID	Protein	Sequence coverage^{^a}	Matched peptides^{^b}	Score^{^c}	Theoretical pI^{^d}	Theoretical Mw^e	Access key^{^f}
Sweet whey (−)
2	β-lactoglobulin	11	2	32	4.93	20.3	P02754
3	β-lactoglobulin	29	6	75*	4.93	20.3	P02754
4	β-lactoglobulin	29	6	75*	4.93	20.3	P02754
5	β-lactoglobulin	17	4	49	4.93	20.3	P02754
6	β-lactoglobulin	11	3	31	4.93	20.3	P02754
7	β-lactoglobulin	19	5	62*	4.93	20.3	P02754
8	β-lactoglobulin	17	4	56	4.93	20.3	P02754
9	FABP^{^g}	37	5	79*	6.14	14.7	P10790
10	α-lactalbumin	28	6	116*	4.93	16.69	P00711
11	α-lactalbumin	22	3	48	4.93	16.69	P00711
12	β-lactoglobulin	11	3	34	4.93	20.3	P02754
13	α_S1-casein	23	3	46	4.89	24.6	P02662
21	BSA^{^g}	16	8	78*	5.80	71.2	P02769
48	Lactophorin/PP3	16	4	52	6.2	17.2	P80195
49	Lactophorin/PP3	25	6	68*	6.2	17.2	P80195
50	Lactophorin/PP3	22	4	49	6.2	17.2	P80195
51	Lactophorin/PP3	39	6	81*	6.2	17.2	P80195
57	Lactophorin/PP3	16	4	52	6.2	17.2	P80195
93	α_S1-CN	23	4	58	4.89	24.6	P02662

The minimum coverage of the matched peptides in relation to the full-length sequence.

The number of matched peptides in the database search.

Score of the Mascot search. Significant scores (P < 0.05) are marked by *.

Theoretical pI of the full-length protein.

Theoretical molecular mass (Mw) of the full-length protein in kg·mol⁻¹.

Primary accession key in the SWISS-PROT database.

FABP and BSA correspond to fatty acid binding protein and bovine serum albumin, respectively.