Different proteomic profiles of sweet whey and rennet casein obtained after preparation from raw versus heat-treated skimmed milk

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Table II.

Proteins in the rennet casein fraction (Figs. 1 and 2) identified by MALDI-TOF MS after non-reducing (−) or reducing (+) 2-DGE.

Spot ID	Protein	Sequence coverage^{^a}	Matched peptides^{^b}	Score^{^c}	Theoretical pI^{^d}	Theoretical Mw^{^e}	Access key^{^f}
Rennet casein (−)
35	α_S1-casein	19	4	61*	4.89	24.6	P02662
37	α_S1-casein	22	4	51	4.89	24.6	P02662
41	α_S2-casein	28	10	85*	4.60	26.0	P02663
45	α_S1-casein	19	4	68*	4.89	24.6	P02662
48	α_S1-casein	19	4	56	4.89	24.6	P02662
56	α_S1-casein	19	4	56	4.89	24.6	P02662
81	β-lactoglobulin	30	6	65*	4.93	20.3	P02754
135	α_S2-casein	25	7	74*	4.60	26.0	P02663
Rennet casein (+)
8	β-lactoglobulin	37	9	106*	4.93	20.3	P02754
10	α-lactalbumin	23	6	65*	4.93	17.0	P00711
11	α_S1-casein	22	4	51	4.89	24.6	P02662
15	α_S1-casein	23	4	49	4.89	24.6	P02662
17	α_S1-casein	22	4	51	4.89	24.6	P02662
18	α_S1-casein	30	6	69*	4.89	24.6	P02662
19	α_S1-casein	22	4	53	4.89	24.6	P02662
22	α_S2-casein	29	8	108*	4.60	26.0	P02663
23	α_S2-casein	18	6	56	4.60	26.0	P02663
26	α_S1-casein	22	4	44	4.89	24.6	P02662

The minimum coverage of the matched peptides in relation to the full-length sequence.

The number of matched peptides in the database search.

Score of the Mascot search. Significant scores (P < 0.05) are marked by *.

Theoretical pI of the full-length protein.

Theoretical molecular mass (Mw) of the full-length protein in kg·mol⁻¹.

Primary accession key in the SWISS-PROT database.