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Table II.
Central composite design matrix and results.
| Experimental no. | Reaction temperature (X 1)/°C | Substrate concentration (X 2)/% | Reaction time (X 3)/h | The decrease of free amino groups (Y)/μmol·g−1 proteins |
|---|---|---|---|---|
| 1 | −1 | −1 | −1 | 10.87 |
| 2 | 1 | −1 | −1 | −31.91 |
| 3 | −1 | 1 | −1 | −16.23 |
| 4 | 1 | 1 | −1 | 7.07 |
| 5 | −1 | −1 | 1 | −6.79 |
| 6 | 1 | −1 | 1 | 14.26 |
| 7 | −1 | 1 | 1 | −34.12 |
| 8 | 1 | 1 | 1 | 9.15 |
| 9 | −1.68 | 0 | 0 | −9.80 |
| 10 | 1.68 | 0 | 0 | −20.64 |
| 11 | 0 | −1.68 | 0 | −8.60 |
| 12 | 0 | 1.68 | 0 | 18.43 |
| 13 | 0 | 0 | −1.68 | −7.74 |
| 14 | 0 | 0 | 1.68 | −4.64 |
| 15 | 0 | 0 | 0 | 78.95 |
| 16 | 0 | 0 | 0 | 81.01 |
| 17 | 0 | 0 | 0 | 80.50 |
| 18 | 0 | 0 | 0 | 80.50 |
| 19 | 0 | 0 | 0 | 79.98 |
| 20 | 0 | 0 | 0 | 79.46 |