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Table I.

Factors and levels of the variables in coded units for RSM study.

Factors Levels
Code −1.682 −1 0 +1 +1.682
Temperature (X 1; °C) 13.2 20 30 40 46.8
Substrate concentration (X 2; %) 29.8 38 50 62 70.2
Reaction time (X 3; h) 1.3 3 5.5 8 9.7