Is microbial diversity an asset for inhibiting Listeria monocytogenes in raw milk cheeses?

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Table II.

PCR primers used for identification of isolates and SSCP analysis.

Primers¹	Species target	Sequences	PCR conditions	Target
W02	Bacterial 16S rDNA	GNTACCTTGTTACGACTT	25 cycles of: 30 s at 94 °C, 30 s at 50 °C, 90 s at 72 °C	SSU 16S rDNA
W18	Bacterial 16S rDNA	GAGTTTGATCMTGGCTCAG	25 cycles of: 30 s at 94 °C, 30 s at 50 °C, 90 s at 72 °C	SSU 16S rDNA
W34	Partial bacterial 16S rDNA	TTACCGCGGCGTGCTGGCAC	30 cycles of: 30 s at 96 °C, 30 s at 61 °C, 30 s at 72 °C	SSU 16S rDNA
W49	Partial bacterial 16S rDNA	ACGGTCCAGACTCCTACGGG	30 cycles of: 30 s at 96 °C, 30 s at 61 °C, 30 s at 72 °C	SSU 16S rDNA
Gram1F	Partial bacterial 16S rDNA	CCTAATACATGCAAGTCG	30 cycles of: 30 s at 96 °C, 30 s at 52 °C, 45 s at 72 °C	SSU 16S rDNA
Gram2R	Partial bacterial 16S rDNA	CTCAGTCCCAATGTGGCC	30 cycles of: 30 s at 96 °C, 30 s at 52 °C, 45 s at 72 °C	SSU 16S rDNA
nl1	Yeast 26S rDNA	GCATATCAATAAGCGGAGGAAAAG	30 cycles of: 30 s at 94 °C, 30 s at 57 °C, 30 s at 72 °C	26S rDNA
nl4	Yeast 26S rDNA	GGTCCGTGTTTCAAGACGG	30 cycles of: 30 s at 94 °C, 30 s at 57 °C, 30 s at 72 °C	26S rDNA
Paracasei 16S	Lactobacillus casei/paracasei	CACCGAGATTCAACATGG	30 cycles of: 60 s at 94 °C, 00 s at 53 °C, 60 s at 72 °C	16S rDNA
16rev	Lactobacillus casei/paracasei	GAAAGGAGGTGATCCAGC	30 cycles of: 60 s at 94 °C, 00 s at 53 °C, 60 s at 72 °C	16S rDNA
16 forward	Lactobacillus plantarum	GCTGGATCACCTCCTTTC	30 cycles of: 60 s at 94 °C, 00 s at 53 °C, 60 s at 72 °C	16S/23S spacer region
Lpl	Lactobacillus plantarum	ATGAGGTATTCAACTTATT	30 cycles of: 60 s at 94 °C, 00 s at 53 °C, 60 s at 72 °C	16S/23S spacer region
16 forward	Lactobacillus curvatus	GCTGGATCACCTCCTTTC	30 cycles of: 60 s at 94 °C, 00 s at 53 °C, 60 s at 72 °C	16S rRNA gene
Lc	Lactobacillus curvatus	TTGGTACTATTTAATTCTTAG	30 cycles of: 60 s at 94 °C, 00 s at 53 °C, 60 s at 72 °C	16S rRNA gene
Lnm1	Leuconostoc mesenteroides/pseudomesenteroides	TGTCGCATGACACAAAAGTTA	25 cycles of: 30 s at 94 °C, 30 s at 62 °C, 90 s at 72 °C	16S rDNA position 185 forward
Lnm2	Leuconostoc mesenteroides/pseudomesenteroides	ATCATTTCCTATTCTAGCTG	25 cycles of: 30 s at 94 °C, 30 s at 62 °C, 90 s at 72 °C	16S rDNA position 470 reverse
Lncit1	Leuconostoc citreum	ACTTAGTATCGCATGATATC	30 cycles of: 30 s at 94 °C, 30 s at 58 °C, 90 s at 72 °C	16S rDNA position 183 forward
Lncit2	Leuconostoc citreum	AGTCGAGTTGCAGACTGCAG	30 cycles of: 30 s at 94 °C, 30 s at 58 °C, 90 s at 72 °C	16S rDNA position 1326 reverse
ddlE1	Enterococcus faecalis	ATCAAGTACAGTTAGTCT	30 cycles of: 60 s at 94 °C, 60 s at 54 °C, 60 s at 72 °C	glycopeptide resistance gene
ddlE2	Enterococcus faecalis	ACGATTCAAAGCTAACTG	30 cycles of: 60 s at 94 °C, 60 s at 54 °C, 60 s at 72 °C	glycopeptide resistance gene
ddlF1	Enterococcus faecium	TAGAGACATTGAATATGCC	30 cycles of: 60 s at 94 °C, 60 s at 54 °C, 60 s at 72 °C
ddlF2	Enterococcus faecium	TCGAATGTGCTACAATC	30 cycles of: 60 s at 94 °C, 60 s at 54 °C, 60 s at 72 °C

References [7, 11, 28].