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Table I.
List of reagents and bonds disrupted by the reagents.
Reagent | Concentration | Bond broken |
---|---|---|
Urea | 3 mol·L−1 | Hydrogena |
DTT | 0.01 mol·L−1 | Sulphydryl |
SDS | 0.1 g·100 g−1 | Hydrophobic (HP) (charge) |
Tween 20 | 0.45 g·100 g−1 | Hydrophobic (HP) (non-ionic) |
Citrate | 0.02 mol·L−1 | Ionic |