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Table I.
List of reagents and bonds disrupted by the reagents.
| Reagent | Concentration | Bond broken |
|---|---|---|
| Urea | 3 mol·L−1 | Hydrogena |
| DTT | 0.01 mol·L−1 | Sulphydryl |
| SDS | 0.1 g·100 g−1 | Hydrophobic (HP) (charge) |
| Tween 20 | 0.45 g·100 g−1 | Hydrophobic (HP) (non-ionic) |
| Citrate | 0.02 mol·L−1 | Ionic |