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Review

Table I.

List of reagents and bonds disrupted by the reagents.

Reagent Concentration Bond broken
Urea 3 mol·L−1 Hydrogena
DTT 0.01 mol·L−1 Sulphydryl
SDS 0.1 g·100 g−1 Hydrophobic (HP) (charge)
Tween 20 0.45 g·100 g−1 Hydrophobic (HP) (non-ionic)
Citrate 0.02 mol·L−1 Ionic
a

Also weakens hydrophobic interactions.