SDS-PAGE patterns (15% acrylamide gel) under reducing conditions of the MFGM material isolated from milk samples obtained before and after evaporation. The processing of MFGM protein isolation was described in Ye et al. [32]. Lane 1, standardised milk; lane 2, pasteurised milk; lane 3, preheated milk; lane 4, DSI-preheated milk; lane 5, after MVR evaporation; and lane 6, after TVR evaporation.