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Figure 2.
Calibration of Superdex® Peptide PE 7.5/300 GFC column using standard peptides. The elution was carried out with 30% of 0.1% TFA in acetonitrile and 70% of 0.1% TFA in water, with a flow rate of 0.25 mL·min−1. Detection was carried out at 215 nm.