Applied PCR cycles for the detection of genus- and species-specific Brucella and differentiation between vaccine and field strains.
|Initial denaturation||No. of cycles||Denaturation||Annealing||Extension||Post-PCR step|
|Genus-specific identification||95 °C for 5 min||40||94 °C for 30 s||56 °C for 30 s||72 °C for 1 min||72 °C for 7 min|
|Differentiation of Brucella species||95 °C for 35 s||45||95 °C for 30 s||58 °C for 30 s||72 °C for 45 s||72 °C for 10 min|
|Differentiation between vaccine and field strains||–||40||95 °C for 1 min||62 °C for 1 min||72 °C for 1.5 min||–|