The electrophoretic profile of the real-time PCR products with the primer pair used for the detection of B. abortus, except for the positive strain. L: DNA ladder (EZ load 100 bp ruler); 1: B. melitensis positive strain; 2–7: DNA of suspected Brucella colonies among which two were isolated from cheese samples (Lanes: 3 and 6 (grown under microaerophilic conditions)), two from Shankleesh (Lanes: 2 (grown under microaerophilic conditions) and 5) and two from Kishk samples (Lanes: 4 and 7); 8: negative control.