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Table II.
Calcium-milk protein interaction determined by ITC – comparison with other investigations.
| Authors (year) |
Technique used |
mol Ca2+/105 g protein |
mg Ca·g−1 protein |
Sample |
pH |
T (°C) |
|---|---|---|---|---|---|---|
| This study | ITC | 20.0 | 8.0 | Whole milk proteins | 7.0 | 25 |
| Pappas and Rothwell [21] | Continuous UF | 24.1 | 9.6 | Whole caseins | 7 | 25 |
| Imade et al. [13] | Gel filtration | 16.0 | 6.4 | 6.9 | 25 | |
| Tessier and Rose [29] | Pressure UF | 33–36 | 13.2 | 7 | 25 | |
| Zittle et al. [33] | Equilibrium dialysis | 29.0 | 11.6 | 7.4 | ||
| Carr [3] in [21] | Membrane electrode | 22.0 | 8.8 | |||
| Chanutin et al. [4] in [21] | Ultracentrifugation | 48.0 | 19.2 | |||
| Pappas and Rothwell [21] | Continuous UF | 44.6 | 17.8 | αs-caseins | 7 | 25 |
| Imade et al. [13] | Gel filtration | 25.0 | 10.0 | 6.9 | 25 | |
| Bingham et al. [2] | Centrifugation | 42.3 | 16.9 | 7 | 37 | |
| Dickson and Perkins [5] | Equilibrium dialysis | 36.0 | 14.4 | 7.4 | 25 | |
| Noble and Waugh [20] | Centrifugation | 46.5 | 18.6 | 7 | 37 | |
| Pappas and Rothwell [21] | Continuous UF | 34.9 | 13.9 | β-caseins | 7 | 25 |
| Imade et al. [13] | Gel filtration | 14.0 | 5.6 | 6.9 | 25 | |
| Dickson and Perkins [5] | Equilibrium dialysis | 20 | 8 | 7.4 | 25 | |
| Pappas and Rothwell [21] | Continuous UF | 22.2 | 8.9 | κ-caseins | 7 | 25 |
| Imade et al. [13] | Gel filtration | 6.0 | 2.4 | 6.9 | 25 | |
| Dickson and Perkins [5] | Equilibrium dialysis | 11.0 | 4.4 | 7.4 | 25 | |
| Pappas and Rothwell [21] | Continuous UF | 35.3 | 14.1 | β-lactoglobulin | 7 | 25 |
| Jeyarajah and Allen [14] | Ca ISE | 8.3 | 3.3 | 7.4 | 25 | |
| Pappas and Rothwell [21] | Continuous UF | 31.0 | 12.4 | α-lactalbumin | 7 | 25 |