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Table I.
Binding parameters obtained from a “one set of sites” fitting model – mean of duplicated experiments.
N | 6 ± 0.1 | mol Ca·mol−1 protein |
N | 8 | mg·Ca·g−1 protein |
K | 1070 ± 15 | mol·L−1 |
ΔH | 1910 ± 38 | cal·mol−1 CC |
ΔS | 20.3 | cal·mol−1 K−1 |
N: number of sites; K: binding constant, depends only on the titrant (CC) concentration; ΔH: heat change, depends only on the titrant (CC) concentration; ΔS: entropy change, calculated from ΔH and K.