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Table I.

Binding parameters obtained from a “one set of sites” fitting model – mean of duplicated experiments.

N 6 ± 0.1 mol Ca·mol−1 protein
N 8 mg·Ca·g−1 protein
K 1070 ± 15 mol·L−1
ΔH 1910 ± 38 cal·mol−1 CC
ΔS 20.3 cal·mol−1 K−1

N: number of sites; K: binding constant, depends only on the titrant (CC) concentration; ΔH: heat change, depends only on the titrant (CC) concentration; ΔS: entropy change, calculated from ΔH and K.