Free Access
Issue
Dairy Sci. Technol.
Volume 88, Number 2, March-April 2008
Page(s) 183 - 191
DOI https://doi.org/10.1051/dst:2007002
Published online 23 February 2008
Dairy Sci. Technol. 88 (2008) 183-191
DOI: 10.1051/dst:2007002

Assessment of the antimicrobial wild-type minimum inhibitory concentration distributions of species of the Lactobacillus delbrueckii group

Morten Danielsen1, Sigrid Mayrhofer2, Konrad Johann Domig2, Ernst Amtmann3, Helmut Karl Mayer3, Ana Belén Flórez4, Baltasar Mayo4, Jenni Korhonen5 and Lorenzo Tosi6

1  Chr. Hansen A/S, Bøge Allé 10-12, 2970 Hørsholm, Denmark
2  Division of Food Microbiology and Hygiene, Department of Food Science and Technology, BOKU - University of Natural Resources and Applied Life Sciences, Vienna, Austria
3  Division of Food Chemistry, Department of Food Science and Technology, BOKU - University of Natural Resources and Applied Life Sciences, Vienna, Austria
4  Instituto de Productos Lácteos de Asturias (CSIC), Villaviciosa, Spain
5  Institute of Applied Biotechnology, University of Kuopio, Finland
6  Institute of Microbiology, Università Cattolica del Sacro Cuore, Piacenza, Italy

(Received 22 June 2007 - Accepted 12 September 2007 - Published online 23 February 2008)

Abstract - Determination of antimicrobial wild-type minimum inhibitory concentration (MIC) distributions is a prerequisite before differentiating susceptible bacteria from bacteria with acquired resistance. The antimicrobial susceptibility was determined for 190 strains of eight species of the Lactobacillus delbrueckii group. These were L. acidophilus, L. amylovorus, L. crispatus, L. delbrueckii, L. helveticus, L. gallinarum, L. gasseri and L. johnsonii. For most antimicrobial agents a clear distinction between susceptible and resistant bacteria was observed. Tetracycline resistance was abundant among L. johnsonii and L. amylovorus strains isolated from animals, while the L. delbrueckii and L. helveticus strains isolated from dairy products, and L. gasseri isolated from humans, rarely contained acquired resistance.


Résumé - Estimation de la distribution des concentrations minimales inhibitrices d'antibiotiques pour des souches sauvages au sein du groupe Lactobacillus delbrueckii
La détermination de la distribution des concentrations minimales inhibitrices d'antibiotiques sur des souches sauvages est un préalable pour différencier les bactéries sensibles de celles qui ont acquis une résistance. La sensibilité aux antibiotiques a été déterminée chez 190 souches de 8 espèces du groupe Lactobacillus delbrueckii  : L. acidophilus, L. amylovorus, L. crispatus, L. delbrueckii, L. helveticus, L. gallinarum, L. gasseri et L. johnsonii. Pour la plupart des antibiotiques, une distinction claire entre bactéries sensibles et bactéries résistantes était observée. La résistance à la tétracycline était abondante parmi les souches de L. johnsonii et de L. amylovorus isolées d'animaux, alors que la résistance acquise des souches de L. delbrueckii et L. helveticus isolées de produits laitiers, et de L. gasseri isolées d'humains, était rare.


Key words: Lactobacillus delbrueckii / Lactobacillus acidophilus / susceptibility testing / food safety / antimicrobial resistance

Mots clés : Lactobacillus delbrueckii / Lactobacillus acidophilus / test de sensibilité / sécurité alimentaire / résistance aux antibiotiques

Corresponding author: dkmda@chr-hansen.com

© INRA, EDP Sciences 2008

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